Validation of quantitative pcr for onion white rot diagnosis
نویسندگان
چکیده
منابع مشابه
Validation of Quantitative PCR Assays
can assess the safety of biotechnology products. Q-PCR precisely quantifies the amount of the nucleic acid target sequence in DNA or RNA extracted from a variety of samples including animal tissues, final products, cell banks, chromatography eluates, and bulk harvest material. During amplification with target-specific oligonucleotide primers, a fluorogenic oligonucleotide probe — with both repo...
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Quantitative fluorescent polymerase chain reaction (QF-PCR) is an accurate and reliable method for rapid detection of aneuploidy; however, it is not routinely used in China. We aimed to validate QF-PCR as a means for prenatal common aneuploidy screening and to analyze the heterozygosities of short tandem repeat (STR) markers in the Chinese population. The sequences of 19 STR markers in chromoso...
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Scedosporium apiospermum and Scedosporium prolificans are fungal pathogens that can cause severe human infections, including disseminated mycosis in immunocompromised patients. Two real-time PCR (RT-PCR) assays for the diagnosis of these species were developed and validated for the classification of clinical strains and for the detection of DNA in clinical samples by use of a murine model of in...
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ژورنال
عنوان ژورنال: New Zealand Plant Protection
سال: 2003
ISSN: 1179-352X,1175-9003
DOI: 10.30843/nzpp.2003.56.6067